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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Res. 2018 Jul 11;78(17):5107–5123. doi: 10.1158/0008-5472.CAN-18-0509

Figure 5: CD201+/EpCAM- cells express pan-keratin but display features of EMT.

Figure 5:

A) Confluence-dependent changes in morphology of CD201+/EpCAM- cells. B) CD24, CD44, CD90, CD201, EpCAM, and pan-keratin staining pattern of cell lines. C) ZEB1 expression levels in various KTB cell lines with and without TGFβ1 treatment. ZEB1 expression in KTB40 and KTB42 was significantly higher than in other KTB cell lines (p<0.0001). D) TWIST1, TWIST2, SNAI1 and SNAI2 expression levels in various KTB cell lines. A TWIST1, TWIST2, SNAI1 and SNAI2 expression in KTB40 and KTB42 was significantly higher than in other KTB cell lines. E. E-cadherin and vimentin expression levels in various KTB cell lines. F) Self-renewal capacity of various cell lines as measured by mammosphere assay. Primary mammospheres of KTB40 and KTB42 but not other KTB cell lines formed tertiary mammospheres. M P1, mammosphere passage 1; M P2 mammosphere passage 2; M P3, mammosphere passage 3. G) Mammosphere forming efficiency of various KTB cell lines. KTB40 and KTB42 cell lines were more efficient than other cell lines in generating mammospheres. H) TP63 and EHF expression levels in various KTB cell lines.