Figure 2.

Extracellular forms are involved in the attenuated cell proliferation induced by LOX transgenesis. (a) Aortic VSMC isolated from wild-type (WT) mice were serum-starved for 24 h and then exposed to conditioned media from transgenic (TgLOX-CM; black bars) or WT VSMC (WT-CM; white bars). Cell proliferation was analyzed by the [³H]-thymidine incorporation method. Results are represented as mean ± SD. *P < 0.002 vs. WT-CM stimulated cells (Mann–Whitney test; n = 6). (b) Endogenous LOX and LOXLs mRNA levels were determined in these cells by real-time PCR. Data are expressed as mean ± SD (Mann–Whitney test; n = 4).