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. 2018 Sep 5;8:13300. doi: 10.1038/s41598-018-31432-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptome analysis strategies and GO enrichment of target DEGs and leaf biomass (a) Venn diagram of down-regulated DEGs of deficient nitrogen condition vs sufficient nitrogen condition in TN90 and TN86 (b) GO enrichment in common down-regulated DEGs between TN90 and TN86 (c) Venn diagram of up-regulated DEGs of deficient nitrogen condition vs sufficient nitrogen condition in TN90 and TN86 (d) GO enrichment in common up-regulated DEGs between TN90 and TN86 (e) Leaf biomass (f) RNA-seq results confirmed by quantitative qRT-PCR. Nd-TN90: TN90 cultivated under deficient nitrogen condition; Ns-TN90: TN90 cultivated under sufficient nitrogen condition; Nd-TN86: TN86 cultivated under deficient nitrogen condition; Ns-TN86: TN86 cultivated under sufficient nitrogen condition. Error bars of leaf biomass indicate standard error of the means (n = 3). Error bars of qRT-PCR indicate standard error of the means (n = 6).