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. 2018 Sep 5;8:13300. doi: 10.1038/s41598-018-31432-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycerol enhanced carbon and nitrogen metabolism in burley tobacco 7 days after spray under different nitrogen levels. Expression levels of nine genes were quantified by qRT-PCR. Carbon metabolism: GLPK (glycerol kinase [Nicotiana attenuata], Gene ID 109221820), gpmA (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase, accession number BX908798), PGK (Phosphoglycerate kinase, Gene ID 107787830), SPS (sucrose-phosphate synthase, Gene ID 107766133), SUS2_2 (sucrose synthase [Nicotiana attenuata], Gene ID 109214879). Nitrogen metabolism: NLP7 (Protein NLP7, Gene ID 828502), NPF3.1 (Protein NRT1/ PTR family 3.1, Gene ID 843186), NPF7.3 (Protein NRT1/ PTR family 7.3, Gene ID 840139), NIA1 (nitrate reductase [NADH] 1 [Nicotiana attenuata], Gene ID 107823732).