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. 2018 Sep 5;8:13300. doi: 10.1038/s41598-018-31432-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of spraying glycerol on total soluble sugar (a,d), reducing sugar content (b,e) and biomass accumulation (c,f) in burley tobacco seedlings under different nitrogen conditions. CK: control; Gl: glycerol. TS: total soluble sugar content. RS: reducing sugar content. Error bars indicate standard error of the means (n = 3). Symbols ** ¹indicate that the significant differences between treatment of control and treatment of spraying glycerol under sufficient nitrogen application (N100) are at 0.01. Symbols ** ²indicate that the significant differences between treatment of control and treatment of spraying glycerol under 20% less nitrogen application condition (N80) are at 0.01 (a–c) TN90 (d–f) TN86.