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. 2018 Aug 30;11:305. doi: 10.3389/fnmol.2018.00305

FIGURE 5.

FIGURE 5

LH and CS concentrations in Nestin- CRHR1 and CRHR2 CKO mice after central CRH infusion. (A) The i.c.v. infusion of ACSF or CRH into the lateral ventricle. (B1) After i.c.v. CRH infusion, LH concentrations were significantly lower in control CRHR1-2LoxP mice (CRH, n = 8 vs. ACSF, n = 7), but not in Nestin-CRHR1 CKO mice (CRH, n = 7 vs. ACSF, n = 7). Two-way ANOVA, F(1, 25) = 13.75, p = 0.010, followed by Bonferroni’s multiple comparisons test (ACSF vs. CRH in CRHR1-2LoxP, ∗p = 0.043, and CRH vs. ACSF in Nestin-CRHR1 CKO, NS, p = 0.7539). Furthermore, after CRH infusion, the LH levels were significantly decreased in the control CHR1-2LoxP mice when compared to the LH levels of Nestin-CRHR1 CKO mice, ∗∗∗p = 0.0004. (B2) After CRH infusion, the CS levels were significantly increased in both groups of animals (either in CRHR1-2LoxP or Nestin-CRHR1 CKO mice) when compared to their respective CS levels after ASCF infusion. Two-way ANOVA, F(1, 25) = 6.67, p = 0.0160, followed by Bonferroni’s multiple comparisons test (CRH vs. ACSF in CRHR1-2LoxP mice, ∗∗∗p < 0.0001 and ACSF vs. CRH in Nestin-CRHR1 CKO mice, ∗∗∗p < 0.0001). Interestingly, the CS levels of the CKO mice were found to be statistically reduced when compared to those of CRHR1-2LoxP mice after CRH infusion (∗∗p < 0.05). (C1) In contrast, after CRH infusion, plasma LH concentrations were found to be significantly decreased in control CRHR2-2LoxP mice (ACSF n = 5 vs. CRH, n = 6), and in Nestin-CRHR2 CKO mice (CRH, n = 6 vs. ACSF, n = 5). Two-way ANOVA, F(1, 18) = 4.477, p = 0.0486, followed by Bonferroni’s multiple comparisons test (CRH vs. ACSF in CRHR2-2LoxP mice, ∗∗p < 0.001, and CRH vs. ACSF in Nestin-CRHR2 CKO mice, ∗∗p = 0.0069). (C2) After CRH infusion, the CS levels were significantly increased in both groups of animals (either in CRHR2-2LoxP or Nestin-CRHR2 CKO mice) when compared to their respective CS levels after ASCF infusion. Two-way ANOVA, F(1, 18) = 96,85, p < 0.0001, followed by Bonferroni’s multiple comparisons test (CRH vs. ACSF in CRHR2-2LoxP, ∗∗∗∗p < 0.0001, and CRH vs. ACSF in Nestin-CRHR2 CKO mice, ∗∗∗∗p < 0.0001).