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. 2018 Aug 30;11:305. doi: 10.3389/fnmol.2018.00305

FIGURE 6.

FIGURE 6

Effect of CRH on LH release from pituitaries of mice lacking CRHR1 or CRHR2. (A1) CRH application induced significant reduction in LH concentrations in the pituitary cultures of WT and CRHR1-KO mice. Two-way ANOVA, F(1, 8) = 9.412, p = 0.0154, followed by Bonferroni’s multiple comparisons test, CRH(+):WT1 vs. CRH(–):WT1, ∗p = 0.0187, and CRH(+):(Global)CRHR1-KO vs. CRH(–):(Global)CRHR1-KO, ∗∗p = 0.0044. (A2) ACTH concentrations were increased in the medium of pituitary cells from WT mice, but not from functional CRHR1-KO mice. Two-way ANOVA, F(1, 8) = 53.21, p < 0.0001, followed by Bonferroni’s multiple comparisons test, CRH(+):WT1 vs. CRH(–):WT1, ∗∗∗p = 0.003, whereas CRH(+):(Global)CRHR1-KO vs. CRH(–):(Global) CRHR1-KO, NS. (B1) CRH application induced significant reduction in LH concentrations in the culture medium of WT mice, but not in that of CRHR2-KO mice. Two-way ANOVA, F(1, 10) = 17.32, p = 0.0020, followed by Bonferroni’s multiple comparisons test, CRH(+):WT vs. CRH(–):WT2, ∗∗p = 0.0031 and CRH(+):(Global)CRHR2-KO vs. CRH(–):(Global)CRHR2-KO, NS, p > 0.05. (B2) ACTH levels in the medium of pituitary cell of WT littermates and global CRHR2-KO mice were equally increased after CRH stimulation in comparison to non-stimulated conditions. Two-way ANOVA, F(1, 10) = 34.75, p = 0.0002, followed by Bonferroni’s multiple comparisons test, CRH(+):WT vs. CRH(–):WT2, ∗∗p = 0.0021, and CRH(+):(Global)CRHR2-KO vs. CRH(–):(Global)CRHR2-KO, NS, p = 0.0065.