Fig. 2.

E4m and E4p are partially redundant in regulating CD4 expression following positive selection. a CD4 and CD8 expression in indicated cell subsets and genotypes. Numbers in the FACS plot quadrants indicate cell percentages. Representative results of more than three independent analyses. b Proportions of CD8 SP cells among TCRβ^hi CD24^loCD69⁻ thymocytes from mice with the indicated genotypes. Mean + SD. ***p < 0.001 (unpaired Student's t-test). c Flow cytometry analysis of ThPOK expression in thymocyte subsets from mice with indicated genotypes. Cells were gated on CD24^loCD69^-TCRβ^hi prior to further gating based on CD8 expression. Data are representative of two independent experiments with multiple mice. d CD4 expression 5 days after in vitro activation and transduction of sorted naive CD4⁺ T cells from Cd4 ^{E4mΔ/E4mΔ E4pFL/FL} mice. Cells were sorted and transduced with retroviral MSCV-Cre-IRES-GFP or control vector. Cells were gated on GFP⁺ transduced cells. Data are representative of two independent experiments with technical replicates. e Quantification of the percentage of cells that lost CD4 expression (CD4^low) after activation and transduction with a retroviral MSCV-Cre-IRES-GFP or control vector. Mean + SD. ***p < 0.001(unpaired Student's t-test)