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. 2018 Sep 5;9:3594. doi: 10.1038/s41467-018-05834-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Cd4 expression during positive selection is critical to prevent lineage re-direction. a CD4 and CD8 expression in indicated cell subsets and genotypes. Numbers in the FACS plot quadrants indicate cell percentages. Representative results of three independent analyses with multiple mice. b CD4 and CD8 expression on indicated cell types, 8 weeks post transfer of Cd4^−/− bone marrow into lethally irradiated β2m^−/− hosts. ** represents un-depleted CD4⁺ T cells in the periphery of β2m^−/− hosts. Experiment was performed three times with two or more mice each time. c Histogram showing Cd4 expression on TCRβ^hiCD24^hiCD69⁺ thymocytes of mice with the indicated genotypes. Data are representative of three independent experiments with multiple mice. d Graph quantifying the MFI of CD4 on recently selected thymocytes from mice shown in c. Data are representative of three independent experiments, with each experiment consisting of two mice in each group. Mean + SD. *p < 0.05, **p < 0.01 (unpaired Student's t-test)