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. 2018 Sep 5;9(9):904. doi: 10.1038/s41419-018-0917-y

Fig. 5. SARS 3a and Rip3 co-localize to the lysosome.

Fig. 5

Confocal imaging analysis from confocal microscopy of (a) HeLa cells expressing LAMP1-CFP, SARS 3a-GFP, and Rip3-mCherry 12 h after transfection. Pearson's correlation coefficient (PCC) between LAMP1-CFP and Rip3-mCherry with and without SARS 3a-GFP is shown on the right (n = 15 cells). b HeLa cells expressing LAMP1-mCherry and SARS 3a-GFP to determine subcellular localization. Pearson's correlation coefficient (PCC) and percent colocalization between LAMP1-mCherry and SARS 3a-GFP are shown on the right (n = 15 cells). ×100 electronic zoom, scale bar 10 μm (***p < 0.0001, Unpaired Student’s T-test)