Table 1.
Absolute quantification of major proteins and protein complexes involved in driving the photosynthetic light reactions in the thylakoid membrane and CO2 fixation in the pyrenoid.
| Protein (complex) | d::pPop rank / score | Peptide | amol/cella | amol/cellb | % of total cell proteinc | |
|---|---|---|---|---|---|---|
| rbcL | 1 / 1 | DTDILAAFR | 24 ± 2.7 | 25.2 | 6.6 | |
| 2 / 0.73 | LTYYTPDYVVR | 30.7 ± 1.8 | ||||
| 3 / 0.68 | FLFVAEAIYK | 22.1 ± 1.6 | ||||
| 5 / 0.6 | (EVTLGFVDLMR | 3.2 ± 0.6)d | ||||
| RBCS1/2 | 1 / 1 | AFPDAYVR | 8.1 ± 2.3 | 16.1 | 1.3 | |
| 2 / 0.996 | AYVSNESAIR | 17.4 ± 1.5 | ||||
| 3 / 0.82 | LVAFDNQK | 21.3 ± 4.1 | ||||
| n.d. | YWTMWK | 13.8 ± 7.6 | ||||
| EPYC1 /LCI5 | 2 / 0.73 | SVLPANWR | 2.2 ± 1.0 | 2.0 | 0.3 | |
| 4 / 0.68 | SALPSNWK | 2.7 ± 1.5 | ||||
| 7 / 0.53 | (TALPADWR | 0.3 ± 0.1) | ||||
| RCA1 | 1 / 1 | VPLILGIWGGK | 0.4 ± 0.1 | 0.5 | 0.1 | |
| 2 / 0.72 | IGQQLVNAR | 0.6 ± 0.1 | ||||
| 11 / 0.32 | SLVDEQENVK | 0.5 ± 0.1 | ||||
| PSII | psbA / D1 | 1 / 1 | VLNTWADIINR | 8.3 ± 4.5 | 5.2 | 2.0e |
| 3 / 0.88 | LIFQYASFNNSR | 3.1 ± 1.1 | ||||
| 7 / 0.52 | EWELSFR | 10.9 ± 5.8 | ||||
| psbD / D2 | 1 / 1 | NILLNEGIR | 6.3 ± 3.2 | |||
| 2 / 0.95 | LVFPEEVLPR | 4.7 ± 1.7 | ||||
| 7 / 0.32 | (TWFDDADDWLR | n.d.) | ||||
| b6/f | petA / cyt f | 1 / 1 | NILVVGPVPGK | 2.3 ± 0.9 | 2.9 | 0.5 |
| 2 / 0.97 | IVAITALSEK | 3.4 ± 1.4 | ||||
| 6 / 0.54 | YPIYFGGNR | 3.5 ± 1.5 | ||||
| PCY1 | 1 / 1 | LGADSGALEFVPK | 9.1 ± 2.7 | 3.5 | 0.2 | |
| 2 / 0.65 | DDYLNAPGETYSVK | 1.3 ± 0.2 | ||||
| PSI | psaB | 1 / 1 | ALYGFDFLLSSK | 3.3 ± 0.7 | 3.1 | 1.3 |
| 3 / 0.95 | TPLANLVYWK | 2.5 ± 1.1 | ||||
| 12 / 0.19 | TNFGIGHR | 3.6 ± 0.7 | ||||
| ATP synthase | atpB | 3 / 0.94 | LSIFETGIK | 4.9 ± 1.3 | 6.1f | 1.6 |
| 7 / 0.72 | TAPAFVDLDTR | 8.0 ± 3.4 | ||||
aMean ± SD, n = 4. bMedian of all values (n = 4 × number of peptides). cBased on median and using MWs of mature proteins. dPeptides in parentheses were not quantotypic and quantification values therefore omitted. eD1 + D2. fThere are three atpB subunits per ATPase complex, i.e., this number needs to be divided by 3 to obtain the number of ATP synthases per cell.