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. 2018 Aug 30;9:1265. doi: 10.3389/fpls.2018.01265

Table 1.

Absolute quantification of major proteins and protein complexes involved in driving the photosynthetic light reactions in the thylakoid membrane and CO2 fixation in the pyrenoid.

Protein (complex) d::pPop rank / score Peptide amol/cella amol/cellb % of total cell proteinc
rbcL 1 / 1 DTDILAAFR 24 ± 2.7 25.2 6.6
2 / 0.73 LTYYTPDYVVR 30.7 ± 1.8
3 / 0.68 FLFVAEAIYK 22.1 ± 1.6
5 / 0.6 (EVTLGFVDLMR 3.2 ± 0.6)d
RBCS1/2 1 / 1 AFPDAYVR 8.1 ± 2.3 16.1 1.3
2 / 0.996 AYVSNESAIR 17.4 ± 1.5
3 / 0.82 LVAFDNQK 21.3 ± 4.1
n.d. YWTMWK 13.8 ± 7.6
EPYC1 /LCI5 2 / 0.73 SVLPANWR 2.2 ± 1.0 2.0 0.3
4 / 0.68 SALPSNWK 2.7 ± 1.5
7 / 0.53 (TALPADWR 0.3 ± 0.1)
RCA1 1 / 1 VPLILGIWGGK 0.4 ± 0.1 0.5 0.1
2 / 0.72 IGQQLVNAR 0.6 ± 0.1
11 / 0.32 SLVDEQENVK 0.5 ± 0.1
PSII psbA / D1 1 / 1 VLNTWADIINR 8.3 ± 4.5 5.2 2.0e
3 / 0.88 LIFQYASFNNSR 3.1 ± 1.1
7 / 0.52 EWELSFR 10.9 ± 5.8
psbD / D2 1 / 1 NILLNEGIR 6.3 ± 3.2
2 / 0.95 LVFPEEVLPR 4.7 ± 1.7
7 / 0.32 (TWFDDADDWLR n.d.)
b6/f petA / cyt f 1 / 1 NILVVGPVPGK 2.3 ± 0.9 2.9 0.5
2 / 0.97 IVAITALSEK 3.4 ± 1.4
6 / 0.54 YPIYFGGNR 3.5 ± 1.5
PCY1 1 / 1 LGADSGALEFVPK 9.1 ± 2.7 3.5 0.2
2 / 0.65 DDYLNAPGETYSVK 1.3 ± 0.2
PSI psaB 1 / 1 ALYGFDFLLSSK 3.3 ± 0.7 3.1 1.3
3 / 0.95 TPLANLVYWK 2.5 ± 1.1
12 / 0.19 TNFGIGHR 3.6 ± 0.7
ATP synthase atpB 3 / 0.94 LSIFETGIK 4.9 ± 1.3 6.1f 1.6
7 / 0.72 TAPAFVDLDTR 8.0 ± 3.4

aMean ± SD, n = 4. bMedian of all values (n = 4 × number of peptides). cBased on median and using MWs of mature proteins. dPeptides in parentheses were not quantotypic and quantification values therefore omitted. eD1 + D2. fThere are three atpB subunits per ATPase complex, i.e., this number needs to be divided by 3 to obtain the number of ATP synthases per cell.