FIGURE 1.

Effects of A_2AR TM peptides on A_2AR–D₂R interactions. (A) The concentration-response effect of the individual A_2AR TM peptides (TM-I^A2A to TM-VII^A2A) on BRET¹ signals of A_2AR–D₂R heteromers. Values represent percentages of maximal BRET¹ responses in cells co-transfected with a 1:1 ratio of D₂R^Rluc and A_2AR^{Y FP} (1 μg cDNA each). Data are averages ± SEM; n = 6 experiments, 6 replicates (A_2AR TM peptides I-III and V-VII); n = 6–10 experiments, 6 replicates (A_2AR TM peptide IV). (B) Comparison of the effects of TM-V^A2A peptide with the vehicle group on the netBRET¹ max values obtained from BRET¹ saturation curves for the A_2AR–D₂R heteromers. Co-transfected cells were incubated with 0.1 μM of TM-V^A2A peptide or vehicle and studied by BRET¹ saturation assays. Data are averages ± SEM; n = 4, eight replicates. ^∗∗∗(TM-V^A2A) significantly different compared to vehicle (P < 0.001) by unpaired t-test.