Fig. 4.

SAC inhibition prolongates TTFields-effects in U-87 MG cells. a Experimental scheme. Application of TTFields (gray background) and IN-3 is indicated in blue and was maintained either alone or in combination for 72 h. TTFields application was ended (EOT red) and cells cultured for another 72 h either with (IN-3, blue) or without (w/o IN-3, red, dotted line) IN-3. Cells were counted after 72, 96, and 144 h overall culture, as indicated. b TTFields were applied to U-87 MG cells for 72 h and then switched off. The cells’ proliferation was determined by cell counting at treatment end (0 h, 100%), and 24 and 72 h after end of TTFields application (w/o TTF, red). U-87 MG cells were incubated with 4 µM IN-3 for 72, 96, and 144 h (IN-3, blue) and counted or incubated with IN-3 for 72 h, further cultivated for 24 and 72 h without IN-3 (w/o IN-3, red, dotted line) and then counted. c For combined treatment, TTFields were applied to U-87 MG cells for 72 h and then switched off (w/o TTF, red), while 4 µM IN-3 was present for 72, 96, and 144 h, respectively (IN-3, blue) or cells were cultured for 72 h with TTFields and 4 µM IN-3 and then TTFields were switched off and IN-3 was removed, while the cells were further cultivated for 24 and 72 h after end of treatment (red, dotted line). Experiments were independently repeated with n ≥ 3. SD is shown as error bars