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. 2018 Sep 5;9(9):906. doi: 10.1038/s41419-018-0902-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Evidence and morphologic observation of VM in HCC. Red arrow points to the PAS-positive and CD31-negative vessels lining with tumor cells. Yellow arrow points to the endothelial cell lined vessels. b Statistical results of MVD and VM of patients treated and untreated with Rhizoma Paridis extract. Results showed reduced MVD and lack of VM in HCC tissues treated by Rhizoma Paridis extract. c, d Immunohistochemical staining of E-cadherin, vimentin, Twist1, VE-cadherin, VEGFR1, VEGFR2, MMP2, MMP9, and laminin5. e Circulating tumor cells in the peripheral blood of patients. The results showed significantly decreased number of CTCs in patients treated with Rhizoma Paridis extract. The values are means of three experiments, and error bars represent standard deviation (*P < 0.05, **P < 0.01)