Figure 3.

Glomerular expression of MHC class II in MsGN models. (A) Immunofluorescence analysis was performed on 4-μm-thick kidney sections to determine MHC class II expression in glomeruli. (B) Quantitative RT-PCR analysis of MHC class II mRNA in glomeruli from MsGN models. (C) Representative Western blots showing the levels of MHC class II protein expression in glomeruli of MsGN models. Data are presented as the mean ± SEM (n = 10 per group). The results are representative of three independent experiments. *P < 0.05 versus Con. Con: non-nephritic phosphate-buffered saline (PBS)-treated animals. Original magnification, × 400; Scale bar = 50 μm.