Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 5;8:13269. doi: 10.1038/s41598-018-31482-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

FLIM-FRET (HEK 293 T). (a) Analysed combinations of fluorescently tagged EpCAM proteins. sfGFP (G) fluorescence is colored cyan and mCherry (R) fluorescence is colored magenta. The same color scheme applies to schematic representations on the left. White line represents 20 µm. FLIM color scale is the same in all presented FLIM measurements. (b) Representative fluorescence lifetime decays for each analysed combination. (c) Mean lifetimes with s.d. of all analysed combinations. For each combination, results obtained in HEK 293 T cells are presented on the left and results obtained in HCT8^EpCAM− on the right, ***p < 0.001, one-way ANOVA test with Bonferroni post hoc analysis, compared to mean values of negative controls. Color scheme is the same as in figure b.