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. 2018 Aug 30;9:2031. doi: 10.3389/fmicb.2018.02031

FIGURE 1.

FIGURE 1

Quantification of the growth and viability of C. glabrata and S. aureus single and mixed biofilms. (A) Biofilm biomass was quantified indirectly by the crystal violet method after 0–48 h of incubation at 37°C for C. glabrata, S. aureus single biofilms and C. glabrata–S. aureus mixed biofilms in which a possible antagonistic interaction was observed due to the significant reduction (∗p < 0.050) of biomass compared with the single biofilm of C. glabrata. (B) Biofilm biomass was quantified after 24 h of incubation at 37°C for C. glabrata, S. aureus single biofilms and C. glabrata–S. aureus mixed biofilms. An evaluation of behavior according to the first colonizer of S. aureus and C. glabrata mixed biofilms was conducted. Fungus or bacterial inoculums were introduced as the first colonizer. (C,D) Viability was evaluated by the CFU assay for C. glabrata and S. aureus in single and mixed biofilm (C) and in single cultures, co-cultures with planktonic cells (D), after 0–48 h of incubation at 37°C. In both cases, the viability of C. glabrata decreased significantly (∗p < 0.050) in co-culture and mixed biofilms with S. aureus compared with the single culture. In all cases, comparisons between absorbance (relative to biomass biofilm formation or CFU) revealed significant differences by the Student–Newman–Keuls or Holm–Sidak tests as appropriate, performing multicomparison of procedures. Values are representative of three experiments with 12 replicates each.