FIGURE 7.

Apoptotic marker detection in C. glabrata with CFBS by CLSM. (A) ROS detection in C. glabrata single biofilms and with CFBS. ROS-positive cells were identified by green fluorescence only in C. glabrata with CFBS. C. glabrata single biofilms did not exhibit any fluorescence. (B) Cell membrane damage detection PI-positive cells were identified by red fluorescence. The images show yeast cell membrane injury caused by CFBS compared with the C. glabrata single biofilm, as evidenced by Calcofluor White, which showed an intact fungal cell wall. The white box inset shows a higher magnification detail. Cell membrane damage in C. glabrata was also examined after exposure to CFBS (B). We did not observe any fluorescence signal in single C. glabrata biofilms that were not exposed to CFBS.