Fig. 1. Schematic diagram of the experimental design used in this study.
Signalling molecules were tested using the Incu Tissue (77 × 77 × 97 mm) system to assess their capacity for inducing SAR against bio- and necrotrophic bacterial pathogens (A). One hundred microlitres each of 1 mM MeSA and MeJA were dropped onto paper discs in one compartment of an I-plate. Twenty microlitres each of serial dilutions (10−6, 10−7 and 10−8) of Pseudomonas syringae pv. tabaci (Pta) and Pectobacterium carotovorum subsp. carotovorum (Pcc) suspensions (OD600 = 1) were dropped onto the surface of the LB medium in the other compartment of an I-plate. Two days later, the number of colonies was counted (B). The experiment was repeated three times with similar results.