Fig. 2. Histochemical analysis of OsWOX13p::GUS reporter gene expression in plant development.

(A) Mature and (B) 2-day imbibed seeds. (C) A 5-day seedling. (D, E) Magnificent of a leaf blade (D) and longitudinal cut through a seed and stem (E) of a 5-day seedling. The white arrow in (E) indicates the position where GUS expression stopped on the crown root. (F) Cross-section of a crown root from a 7-day seedling. (G) Lateral root initiation in a 7-day seedling crown root. (H, I) Junctions with developed (H) and developing (I) lateral root in a 7-day seedling radicle. (J) Six-week-old stem with all developed leaves removed. The arrow shows the position of an auxiliary tiller where it is cross cut (K) and 12-μm-sectioned (L). The section in (L) was stained with toluidine blue. (M) The 12-μm cross-section of the SAM sample was stained with toluidine blue. (N) Hand section of a node from an unelongated stem. (O) Hand section of node N^th counted upward along an elongated stem. In node N^th, DVs only surround and connect to enlarged large VBs of leaf N^th at NVA. VBs of leaf (N+1)^th are not enlarged and only pass through the node. (P) Panicles at the very early stage of inflorescent formation. (Q to T) Floret organs at stage (Q) 1 cm, (R) 12 cm, and (S) before and (T) after pollination. (U, left to right) Seeds at 2, 4, 6, 8, and 10DAP. Scale bar = 0.2 cm (a, b, c, e, j, t); 50 μm (F, H) or 200 μm (others). sc: scutellum, pl: primary leaf, ra: radicle, cr: crown root, me: mesocotyl, mv: main vien, NVA: nodal vascular anatomoses, VBs: vascular bundles, P_LN and X_LN: phloem and xylem of enlarged large VBs of leaf N^th, S_N: enlarged small VBs of leaf N^th, L/S_N+1: large/small VBs of leaf (N+1)^th, DVs: diffuse vascular bundle, SAM: shoot apical meristem, IM: inflorescence meristem, DAP: day-after-pollination.