Fig. 5. Overexpression of OsWOX13 (rab21 promoter) showed early flowering.
(A) Three T3lines showed the early flowering phenotype in the field. Wild type Ilmi showed heading around Aug. 17th in the irrigated field, and the transgenic plants showed 7–10-d earlier flowering compared with Ilmi. (B) Normal distribution of heading dates of transgenic and wild type plants. (C) Mature leaves were collected at day 40, 33, 25 (Δ40, Δ33, Δ25; respectively) prior to the heading date. The 1 cm (P1cm, Δ19) and 8 cm (P8cm, Δ12)-long panicles were also included. The transgenic line 8-4-1 was used as a representative (OsWOX13-ov). (D) The expression level of flowering-related genes by qPCR with tubulin as the expression standard.