Table 9.
Differentially regulated annotated transcripts in microgravity identified in the 19th DLR parabolic flight and TEXUS-49 suborbital ballistic rocket campaigns.
| Gene name | Full gene name | Probeset ID | Gene function adopted from GeneCards HUMAN GENE DATABASE | Fold change 19th DLR PFC µg vs BL-PFC hyp-g | Fold change TEXUS-49 µg vs BL-TX hyp-g |
|---|---|---|---|---|---|
| AKNA | AT-hook transcription factor | AB075848 | RNA polymerase II core promoter proximal region sequence-specific DNA binding and transcriptional activator activity | 1.627 | 1.670 |
| LOC390988 | hypothetical LOC390988 | XM_372755 | unknown function | 1.655 | 1.524 |
| L3MBTL2 | l(3)mbt-like 2 (Drosophila) | AL136564 | putative polycomb group (PcG) protein, maintains the transcriptionally repressive state of genes, involved in histone binding | 1.414 | 1.610 |
| TLN1 | talin 1 | BC020881 | cytoskeletal protein that accumulates at cell-substratum and cell-cell contact sites, involved in actin filaments and assembly and cell spreading and migration, colocalisation with integrins in cell surface membranes enabling the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells | 1.330 | 1.806 |
| VPS29 | vacuolar protein sorting 29 (yeast) | BC032462 | large multimeric retromer complex component, plays a role in retrograde transport of proteins from endosomes to the trans-Golgi network | 1.334 | 1.332 |
| EPHA6 | EPH receptor A6 | AY358738 | receptor tyrosine kinase binding GPI-anchored ephrin-A family ligands on adjacent cells, important for G-protein signaling RhoA regulation pathway, and EPHA forward signaling | −1.404 | −1.397 |
| FCRLM1 | Fc receptor-like and mucin-like 1 | AF329495 | receptor similar to receptors for the Fc fragment of gamma immunoglobulin | −1.336 | −1.322 |
| PCYT1B | phosphate cytidylyltransferase 1, choline, beta | NM_004845 | regulation of phosphatidylcholine biosynthesis | −1.319 | −1.511 |
| LOC643980 | similar to FRG1 protein (FSHD region gene 1 protein) | XM_928714 | localizes to the nucleolus, involved in actin filament binding, poly(A) RNA binding, mRNA transport, and epigenetic regulation | −1.386 | −1.757 |
Annotated transcripts with continuous response for up or down-regulation after 20 s and 300 s of microgravity are shown. Positive sign: significantly up-regulated transcripts (p-value <0.05, FC ≥+1.3), negative sign: significantly down-regulated transcripts (p-value < 0.05, FC ≤−1.3). FCs are ratios between the averages of linear expression values. If the ratio is <1, FC is calculated as the negative reciprocal of the ratio.