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. 2018 Jul 28;109(9):2781–2791. doi: 10.1111/cas.13715

Figure 2.

Figure 2

Effects of siRNA‐mediated inhibition of ClC‐3, ClC‐7, and chloride intracellular channel protein 1 (CLIC)1 on expression levels of human epidermal growth factor receptor 2 (HER2) transcripts in MDA‐MB‐453 cells. A, Real‐time PCR assay for HER2 in control siRNA (si‐cont)‐, ANO1 siRNA (si‐ANO1)‐, ClC‐3 siRNA (si‐ClC‐3)‐, ClC‐7 siRNA (si‐ClC‐7)‐, and CLIC1 siRNA (si‐CLIC1)‐transfected MDA‐MB‐453 cells for 72 h. Expression levels were expressed as a ratio to β‐actin (ACTB). B‐D, Protein lysates of si‐cont‐, si‐ANO1‐, si‐ClC‐3‐, si‐ClC‐7‐, and si‐CLIC1‐transfected MDA‐MB‐453 cells were probed by immunoblotting with anti‐HER2 and anti‐ACTB antibodies on the same filter (B, C). Summarized results were obtained as described in Section 2.5 from HER2 and ACTB band signals (D). After compensation, the HER2 signal in the si‐cont group was expressed as 1.0 (n = 4 for each). Results are expressed as means ± SEM. **P < .01 vs si‐cont. ANO, anoctamine