Figure 5.

Effects of siRNA‐mediated inhibition of ClC‐3 for 72 h on the expression levels of phosphorylated AKT (P‐AKT) and phosphorylated STAT3 (P‐STAT3) proteins in MDA‐MB‐453 cells. A, B, Protein lysates of control siRNA (si‐cont) and ClC‐3 siRNA (si‐ClC‐3)‐transfected MDA‐MB‐453 cells were probed by immunoblotting with anti‐P‐AKT, anti‐AKT, and anti‐ACTB (A) or anti‐P‐STAT3, STAT3, and ACTB (B). After compensation, the ratio of P‐AKT to AKT or P‐STAT3 to STAT3 in si‐cont was expressed as 1.0 (n = 6 for each). Results are expressed as means ± SEM. **P < .01 vs si?cont. ACTB, β‐actin; STAT, signal transducer and activator of transcription