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. 2018 Jul 28;109(9):2781–2791. doi: 10.1111/cas.13715

Figure 6.

Figure 6

Distribution of ClC and chloride intracellular channel protein (CLIC) subtype transcripts and effects of siRNA‐mediated inhibition of YMB‐1 cells. A, B, Real‐time PCR assay for 7 ClC subtypes (ClC‐1‐ClC‐7) (A) and 6 CLIC subtypes (CLIC1‐CLIC6) in YMB‐1 cells (n = 3 for each). C, Real‐time PCR assay for human epidermal growth factor receptor 2 (HER2) in control siRNA (si‐cont)‐, ClC‐3 siRNA (si‐ClC‐3)‐, ClC‐7 siRNA (si‐ClC‐7)‐, and CLIC1 siRNA (si‐CLIC1)‐transfected YMB‐1 cells for 72 h. Expression levels are expressed as a ratio to β‐actin (ACTB). D, Protein lysates of si‐cont‐, si‐ClC‐3‐, si‐ClC‐7‐, si‐ANO1‐, and si‐CLIC1‐transfected YMB‐1 cells were probed by immunoblotting with anti‐HER2 and anti‐ACTB antibodies on the same filter (Figure S2A,B). After compensation, the HER2 signal in the si‐cont group was expressed as 1.0 (n = 4 for each). Results are expressed as means ± SEM. **P < .01 vs si‐cont