Figure 9.

Effects of siRNA‐mediated inhibition of ClC‐3 and ANO1 on expression levels of human epidermal growth factor receptor 2 (HER2)‐related transcriptional factor transcripts in MDA‐MB‐453 and YMB‐1 cells, respectively. Real‐time PCR assay for AP‐2α (A, H), AP‐2β (B, I), AP‐2γ (C, J), STAT3 (D, K), EBP1 (E, L), MBP‐1 (F, M), and HDAC6 (G, N) in control siRNA (si‐cont) and ClC‐3 siRNA (si‐ClC‐3)‐transfected MDA‐MB‐453 cells for 72 h (A‐G) and ANO1 siRNA (si‐ANO1)‐transfected YMB‐1 cells for 72 h (H‐N), respectively. Expression levels were expressed as a ratio to ACTB (n = 4 for each). Results are expressed as means ± SEM. ANO, anoctamine; AP, activator protein; EBP, ErbB3‐binding protein; HDAC, histone deacetylase; MBP‐1, major basic protein 1; STAT, signal transducer and activator of transcription