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. 2018 Sep 5;1:134. doi: 10.1038/s42003-018-0131-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — IDLV DNA persists at the site of injection at 6 months post immunization. a Total-IDLV DNA PCR and β-actin PCR were performed using the indicated DNA amounts extracted from the muscle biopsies. Genomic DNA (5 ng) extracted from the positive control (CMMT-LV-Neo) corresponding to 5 × 10² cells was also amplified. b Five PCR replicates were performed for each sample to increase the probability of detecting IDLV DNA in all the animals