Table 2.
mAb neutralization assay against the indicated viruses
| Neutralization panel | ||||||
|---|---|---|---|---|---|---|
| Ab ID | Binding specificity | SVA-MLV (Neg. ctrl) | MW965.26 (tier 1) | Ce1086_B2 (tier 2) | Ce1176_A3 (tier 2) | 25710-2.43 (tier 2) |
| 910893 | V1V2 | >50 | >50 | >50 | >50 | >50 |
| 910894 | CD4bs | >50 | >50 | >50 | >50 | >50 |
| 910895 | V1V2 | >50 | >50 | >50 | >50 | >50 |
| 910900 | V1V2 | >50 | >50 | >50 | >50 | >50 |
| 911883 | V1V2 | >50 | >50 | >50 | >50 | >50 |
| 911884 | V1V2 | >50 | >50 | >50 | >50 | >50 |
| 911890 | gp120 | >50 | 0.05 | >50 | >50 | >50 |
| 911892 | V3 | >50 | <0.02 | >50 | >50 | >50 |
| 911897 | CD4bs | >50 | 0.89 | >50 | >50 | >50 |
| 911899 | CD4bs | >50 | >50 | >50 | >50 | >50 |
| 911900 | gp120 | >18.5 | 0.57 | >18.5 | >18.5 | >18.5 |
| 911901 | V3 | >50 | <0.02 | >50 | >50 | 13.53 |
| 911904 | CD4bs | >50 | 26.59 | >50 | >50 | >50 |
| 911905 | V1V2 | >50 | >50 | >50 | >50 | >50 |
Values are the antibody concentration in µg mL−1 at which relative luminescence units (RLUs) were reduced 50% compared to virus control wells (no test sample) after subtraction of background RLUs in cell control wells. A response was considered positive if the IC50 was 3 times greater than the signal against the MLV-pseudotyped negative control virus. The bold values indicate a positive response. The binding specificity on the HIV-1 envelope is shown for each mAb