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CD274/PD‐L1 gene amplification by FISH analysis from 2 patients with colorectal cancer. CD274 (red) and CEN9 (green) on chromosome p24.1 (magnification, ×1000). Representative images obtained from the same patients show programmed cell death ligand‐1 protein expression in tumor cells (intensity scores: assay 1, 2+; assays 2 and 3, 3+) (magnification, ×200)
