Fig. 2. A-1210477 combined with ABT-263 killed the MIC population of melanoma cells regardless of the mutation status.

Melanoma cells were subjected to the primary sphere assay. Spheres were treated with indicated compounds either alone, or in combination, of the same concentration as in Fig. 1b for 48 h, and were then subjected to a bright field analysis, scale bar = 100 μm and b quantification of the number of primary spheres. For clarity, we marked only the combinational treatments that were significantly different from comparisons with the DMSO and the single drug treatments. Within each significant combination treatment, we only show the least significant p-value of the comparisons. ** indicates p < 0.01; *** indicates p < 0.001. c Protein lysates were prepared under the same treatment conditions and were probed for PARP. Molecular weight markers are in kDa