Phylogenetic tree based on MLST of H. influenzae clinical isolates. A neighbor-joining dendrogram of BLNAS (n = 10), low-BLNAR (n = 5), high-BLNAR (n = 54), BLPAR (n = 5), and BLPACR (n = 2) isolates investigated in this study was constructed. Four isolates (SMHi5, SMHi67, SMHi97, and SMHi102) were excluded because fucK was not amplified by PCR. Dotted rectangles indicate the most common type of amino acid substitution in group III, and solid rectangles indicate the second most common type. Asterisks indicate singletons.