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MIC values for colistin and polymyxin against E. coli strain DH5α carrying the wild-type mcr-3 gene or either of the two mutation variants (Thr277Ala and Thr277Ser)
| E. coli strain | MIC (μg/ml) |
|
|---|---|---|
| Colistin | Polymyxin | |
| DH5α | 0.5 | 0.5 |
| DH5α(pUC19) | 0.5 | 0.5 |
| DH5α(pUC19-mcr-3) | 4 | 4 |
| DH5α(pUC19-mcr-3) (Thr277Ala) | 0.5 | 0.5 |
| DH5α(pUC19-mcr-3) (Thr277Ser) | 1 | 1 |
