TABLE 2.

Mutation types found in USUV populations treated with different drugs

Druga	No. of mutations in each population	No. of nucleotides sequenced in each population	No. of mutations by typeb					Transition frequency (10 × −4)c
			A to G	U to C	G to A	C to U	Tv	A to G	U to C	G to A	C to U
No drug	8	45,337	2	1	2	0	3	1.8	1.0	1.6	<0.9
DEC	8	33,947	1	2	0	2	3	1.2	2.6	<1.1	2.4
FAV	20	34,218	3	5	5	6	1	3.5	6.4	5.2	7.2
RBV	11	25,897	0	2	4	4	1	<1.5	3.4	5.5	6.4
FU	37	34,453	10	15	1	3	8	11.5	19.2	1.0	3.6

^aUSUV populations were serially passaged five times in the absence of drug (no drug) or in the presence of either decitabine (DEC), favipiravir (FAV), ribavirin (RBV), or 5-fluorouracil (FU) at a concentration of 800 μM.

^bDifferent type of mutations found in the analysis. Given are the number of times that each transition type is found in the analysis. Tv indicates the number of transversions found in the analysis.

^cTransition frequencies found in populations treated with mutagenic drugs. These numbers have been normalized to the nucleotide composition in the sequenced amplicon. Highlighted in bold is the most frequent transition in each sample.