TABLE 1.

Primers for genotyping pfcrt, pfmdr1, and k13-propeller genes

Gene (ID)	PCR round	Primer	Sequence (5′–3′)	Primer binding region		Size (bp)
				Start	End
pfcrt (PF3D7_0709000)	Primary	Pfcrt_Outer P1	CCGTTAATAATAAATACACGCAG	−86	−64	547
		Pfcrt_Outer P2	CGGATGTTACAAAACTATAGTTACC	436	460
	Secondary	Pfcrt_Inner P1	TGTGCTCATGTGTTTAAACTT	307	327	145
		Pfcrt_Inner P2	CAAAACTATAGTTACCAATTTTG	429	451
pfmdr1 (PF3D7_0523000)	Primary	Pfmdr1(1)-N1F	TTAAATGTTTACCTGCACAACATAGAAAATT	137	167	612
		Pfmdr1(1)-N1R	CTCCACAATAACTTGCAACAGTTCTTA	722	748
	Secondary	Pfmdr1(1)-N2F	TGTATGTGCTGTATTATCAGGA	183	204	526
		Pfmdr1(1)-N2R	CTCTTCTATAATGGACATGGTA	687	708
	Primary	Pfmdr1(2)-N1F	AATTTGATAGAAAAAGCTATTGATTATAA	3019	3047	880
		Pfmdr1(2)-N1R	TATTTGGTAATGATTCGATAAATTCATC	3871	3898
	Secondary	Pfmdr1(2)-N2F	GAATTATTGTAAATGCAGCTTTA	3068	3090	799
		Pfmdr1(2)-N2R	GCAGCAAACTTACTAACACG	3847	3866
k13-propeller (PF3D7_1343700)	Primary	PfK13_outF	GGGAATCTGGTGGTAACAGC	65	84	2,097
		PfK13_outR	CGGAGTGACCAAATCTGGGA	2142	2161
	Secondary	PfK13_inF2	TCAACAATGCTGGCGTATGTG	1398	1418	501
		PfK13_inR2	TGATTAAGGTAATTAAAAGCTGCTCC	1873	1898