FIG 1.

Inhibition of LmPBPD2 with three penams (A) and three cephems (B). The y axis represents the relative intensity of the Bocillin FL fluorescence, while the x axis represents the logarithmic concentration of the antibiotic. The IC₅₀ values were determined via SDS-PAGE-based Bocillin FL competition assays. Error bars indicate standard deviations based on three independent experiments. IC₅₀ values represent the concentration of unlabeled antibiotics required to reduce the binding of Bocillin FL by 50%. The chemical structures of the β-lactam antibiotics that were used in the present study are shown above the graphs.