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. 2018 Sep 5;8:13297. doi: 10.1038/s41598-018-31543-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasmid curing alters the volatile metabolome of isolates belonging to ST258. Box plots depict relative compound abundance for metabolites that were: (1) identified as discriminatory between ST258 and non-ST258 by RF, linear SVM, and PLS-DA, and (2) significantly different in relative compound abundance between wild-type ST258 isolates and cured ST258 isolates (p < 0.05 after Benjamini-Hochberg correction). Red: ST258 WT; purple: ST258 cured; blue: non-CP (all STs); dark red: CP isolates (all other STs). The y-axis (in arbitrary units [a.u.]) corresponds to relative compound abundance, as measured by total ion chromatogram (TIC), following log₁₀-transformation, mean-centering, and unit-scaling.