Fig. 5.

Pectolinarigenin inhibits tumor growth, metastasis, and prolongs survival in an orthotopic osteosarcoma mouse model. a 143B cells were injected into the medullary cavity of tibia of the tested mice. 24 days-post pectolinarigenin administration, mice in different groups were sacrificed and the posterior limb with tumors was weighed (*P < 0.05; ***P < 0.001). b Lungs in different groups were excised and weighed (*P < 0.05; ***P < 0.001). c Lung colonization was visualized by a dissecting microscope and lung metastasis nodules were counted manually (***P < 0.001). Four micrometer sections of lungs were subject to H&E staining (left lower panel). Scale bar, 100 μm. d Overall survival rate in the orthotopic osteosarcoma mouse model. e Primary tumors were removed, fixed, and paraffin embedded at the end of the experiment. Four micrometer sections were analyzed by IHC using an anti-phospho-STAT3 (Y705) antibody. Scale bar, 100 μm. f Primary tumors were lysed and subjected to immunoblotting with indicated antibodies. Actin was used as a loading control