Fig. 3. CD69 over-expression enhances IL-10 expression.

1 × 10⁶/ml EL4 cells were cultured in the supernatants from Plat-E cells that had transfected with control retrovirus pMX or pMX-CD69 at an MOI of 50 for 48 h. a The levels of CD69 were determined using flow cytometry. b, c Il-10 and TGF-β1 expression in EL4 cells treated as indicated were detected by real-time PCR (n = 3). d, e The levels of IL-10 and TGF-β1 in the supernatants of cultured cells as described in b and c were determined by ELISA (n = 3). Date are representative images or expressed as the mean ± SD of three independent experiments. Statistical significance is indicated by ANOVA or Student’s t-test. **P < 0.01, ns, no significant