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. 2018 Sep 5;8:13266. doi: 10.1038/s41598-018-31480-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The MTBD made contacts (in logarithmic scale) along the length of E-hook B as the MTBD approached the microtubule. Bars represent the average number of contacts made by each E-hook B residue and the MTBD at MTBD-microtubule distances of 0, 5, 15, 25, 35, and 45 Å (panels a–f, respectively). Three independent runs (see Methods) were analyzed and results averaged for each distance, and 2000 total frames were analyzed for each run.