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. 2018 Sep 5;8:13252. doi: 10.1038/s41598-018-31621-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Active IP3R are recruited to the STIM1 puncta upon activation. (A) Typical confocal microscopy images illustrating the localization of the YFP-IP3R (shown in red) and STIM1-CFP (shown in green) at the endoplasmic reticulum in cells stimulated with bradykinin (Bk). (B) Amplification of the areas shown in A with a white rectangle to show in greater details the puncta formation for YFP-IP3R (shown in red) and STIM1-CFP (shown in green). To the right are the co-localization panels obtained with Imaris before and after bradykinin (Bk) stimulation. (C) Typical confocal microscopy images illustrating the localization of the YFP-IP3R (shown in red) and STIM1-CFP (shown in green) at the endoplasmic reticulum in cells stimulated with thapsigargin (TG). (D) Amplification of the areas shown in A with a white rectangle to show in greater details the puncta formation for YFP-IP3R (shown in red) and STIM1-CFP (shown in green). To the right are the co-localization panels obtained with Imaris before and after thapsigargin (TG) stimulation. R represents the Pearson correlation coefficient. Co-localization pixel by pixel was mapped to the pseudo color scale illustrated to the right from 0 (blue) to 1 (red). Scales show 5 microns (μ) for panel A and 6 microns (μ) for panel B.