Figure 1.

hnRNP A1 iCLIP reads at IKBKAP exon 20. (A) Analysis of the splicing of IKBKAP exon 20 in FD-derived fibroblasts (GMO4589) and control fibroblasts (GMO2912). FD-derived fibroblasts contain partially skipped IKBKAP exon 20 and reduced expression levels compared with control fibroblasts. (B) Screenshot from the IGV genome browser. Upper: hnRNP A1 iCLIP reads (gray) surrounding IKBKAP exon 20. Lower: enlargement of the 5′ splice site region. The iCLIP technique results in reads that begin at or close to the cross-linked nucleotide. As such, the beginning of the reads indicates the most likely location of hnRNP A1 binding. Two hnRNP A1 binding motives (underscored) are present downstream of the 5′ splice site overlapping with the iCLIP reads. IKBKAP is located on the reverse strand.