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. 2018 May 14;46(15):7938–7952. doi: 10.1093/nar/gky395

Figure 3.

Figure 3.

SPRi analysis confirms hnRNP A1 binding to the IKBKAP SSs. SPRi of recombinant hnRNP A1 protein binding to biotinylated RNA oligonucleotides harboring WT and mutant ESS1, ESS2 and ISS. Protein was injected in increasing 2-fold concentrations from 6.25 to 200 nM. ISS and ESS2 oligonucleotide measurements were fitted to a bimodal 1:2 binding model, to account for multiple hnRNP binding sites on the oligonucleotide. ESS1 oligonucleotide measurements could only be fitted to a monomodal 1:1 binding model. KD is based on kd/ka. It is the best indicator of how much protein the oligonucleotides can bind at equilibrium. It was not possible to calculate KD for ISS MUT and ESS1 MUT due to low binding.