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. 2018 May 14;46(15):7938–7952. doi: 10.1093/nar/gky395

Figure 4.

Figure 4.

hnRNP A1 and hnRNP A2/B1 regulate inclusion of IKBKAP exon 20. Knockdowns of hnRNP A1 or hnRNP A2/B1 in four different FD patient fibroblasts GMO4663, GMO4589, GMO4899 and GMO2343. (A) hnRNP A1 knockdown increases IKBKAP exon 20 inclusion, while hnRNP A2/B1 decreases IKBKAP exon 20 inclusion. (B) IKBKAP exon 20 inclusion was quantified using the fragment analyzer. Error bars indicates SEM, n ≥ 2 ***P-value < 0.001, **P-value < 0.01, *P-value < 0.05 knockdown was validated by qPCR where expression of hnRNP A1 (C) and hnRNP A2/B1 (D) was normalized to the expression of TBP. Error bars indicates SEM, n ≥ 3 ***P-value < 0.001, **P-value < 0.01, *P-value < 0.05. (E) Knockdown of hnRNP A1 and hnRNP A2/B1 was confirmed by western blotting (GMO4663) and HPRT was used as a loading control.