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. 2018 Jun 25;46(15):7662–7674. doi: 10.1093/nar/gky536

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Nuclear association of EVI1 with CtBP1. (A) EVI1 (green) and CtBP1 (red) immunofluorescence signal of HEK293T cells transfected with EVI1-WT (upper panel) and EVI1-AQA mutant (middle panel), or SB1690CB AML cells (lower panel) after genotoxic stress induced either with H₂O₂ treatment or irradiation (2 Gy). Left top corner inserts represent 4 μm² regions of interests (ROI) in HEK293T cells or 3 μm² ROIs in SB1690CB cells (eight ROIs/cell analyzed). (B) Quantification of EVI1 and CtBP1 co-localization in EVI1-WT or EVI1-AQA transfected HEK293T cells after genotoxic stress. Degree of co-localization is expressed as Pearson's Coefficient (P’sC). Each bar represents the average P’sC of independent experiments (n = 3) for H₂O₂ (left panel) and irradiated cells (right panel). (C) Distribution of P’sC for endogenously expressed EVI1 and CtBP1 co-localization in SB1690CB leukemia cells after H₂O₂ treatment or irradiation. Each dot represents a single cell (left panel). Average P’sC of independent experiments (n = 3) plotted as bar graphs (right panel). Statistical analysis: one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant).