Table 1.
Summary of determined diffusion coefficients
| Protein fusion/genotype |
MM (kDa) |
Diffusion coefficient [μm2 s−1 ± SD*] |
Number of tracks | Fraction (%) | Sets | Tracks per set |
|---|---|---|---|---|---|---|
| Smc-YFP | 158.8 | D1: 0.53 ± 0.12 | 1312 | 52 ± 5 | 3 | 345; 561; 406 |
| D2: 0.10 ± 0.01 | 47 ± 6 | |||||
| ScpA-YFP | 56.6 | D1: 1.32 ± 0.32 | 689 | 43 ± 10 | 3 | 213; 145; 331 |
| D2: 0.10 ± 0.01 | 57 ± 9 | |||||
| ScpB-YFP | 49.0 | D1: 1.02 ± 0.25 | 972 | 38 ± 12 | 4 | 243; 114; 312; 303 |
| D2: 0.10 ± 0.01 | 62 ± 13 | |||||
| ScpA-YFP, smc::kan, amyE::pspac-smc | 56.5 | 1.43 ± 0.35 | 858 | 100 | 3 | 321; 337; 200 |
| ScpA-YFP, scpB::kan, amyE::pspac-scpB-cfp | 56.5 | 3.22 ± 1.41 | 867 | 100 | 3 | 477; 213; 177 |
| headless-mVenus | 124.0 | 0.40 ± 0.09 | 768 | 100 | 3 | 245; 166; 357 |
| mVenus | 27.0 | 4.9 ± 0.92 | 399 | 100 | 4 | 112; 138; 98; 51 |
*Fitting to data from a set was done with a model of two populations, with a constrained fit for one population at D = 0.1 μm2 s−1. An F-test justified the fitting of two populations for these fusions, whereas it justified fitting of one population for example for mVenus. SD refers to the standard deviation from fitting of the amount of sets indicated, so at least three independent replicates (sets) were used for the determination of the mean and standard deviation of each diffusion coefficient.