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. 2018 Jul 9;46(15):7886–7901. doi: 10.1093/nar/gky601

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Alt-P3 may promote but is not essential for active telomerase assembly in in cells. (A) Activity assay, (B) immunoblot and (C) northern blot to follow assembly and activity of P3 duplex-swap hTRmin mutations co-expressed with ZZF-tagged TERT in U20S cells, paralleling the experiments described in Figure 5. Values for rRAP* and rAct were calculated as described in Figure 3. Similar results were obtained from two independent experimental replicates.