Figure 1.

Identification of central channel Nucleoporins Nup54, Nup62 and Nup58 as novel radioprotecting factors. (A) Mean R-scores values for the genes in the secondary screen ordered by rank product. A negative R-score denotes radiosensitization. (B–E) CFAs performed in HeLa, T24, BT549, MCF7 and DU145 cells following siRNA transfection with the indicated siRNAs. P-values < 0.05 were considered significant (ANOVA; n = 3 technical replicates from a representative experiment repeated at least three times). Nup54 knockdown was confirmed by western blotting 72 h after transfection (β-actin level was used as a loading control).