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. 2018 Jul 9;46(15):7731–7746. doi: 10.1093/nar/gky569

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Nup54 KD does not influence the cell-cycle progression of cells irradiated in G1. (A) Hela cells were synchronized in G1/S by DT block, either left untreated or exposed to 4 Gy IR, and then released from the DT block and incubated with nocodazole for mitotic arrest. Cells were analysed by flow cytometry for DNA content (PI) and p-H3. Graph depicts the percentage of mitotic cells as a function of time. (B) Representative overlapping histograms (top panel) and dot-plots (bottom panel) from the experiment described in A. Values shown in graph correspond to mean ± sd from three independent experiments.