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. 2018 May 18;46(15):7924–7937. doi: 10.1093/nar/gky394

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — RNA binding results in assembly of a larger σNS oligomer. (A) Size-exclusion chromatography elution profiles of σNS apoprotein (blue) and σNS–RNP complex (red). Absorbances at 260 and 280 nm are shown as dashed and continuous lines, respectively. DLS-derived hydrodynamic radii (R_h) are shown for each species. A second, minor peak corresponds to free, excess RNA. (B) SAXS curves for σNS apoprotein (blue) and RNP complex (red), with respective fits shown in black. Scattering profiles are shown as the logarithm of the scattering intensity, I, as a function of the momentum transfer, q = 4πsin(θ)/λ. Radii of gyration (R_g) values of both species are displayed. (C) Normalized pair-wise distance distribution functions, P(r), calculated from the scattering curves of σNS apoprotein (blue) and RNP complex (red) showing an increase in maximum dimension (D_max).