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σNS RNP complex is predominantly octameric. (A and B) Native ESI-MS of σNS apoprotein (A) and RNP complex (B). Averaged collision cross-sections, CCS (Ω) are shown in nm2 for each species. Inset: relative abundances of hexameric and octameric σNS oligomers. Smaller protein oligomers observed in both spectra are due to dissociation of higher order species during the ionization process.
